NCJ Number
205137
Journal
Journal of Forensic Sciences Volume: 49 Issue: 2 Dated: March 2004 Pages: 260-262
Date Published
March 2004
Length
3 pages
Annotation
This article describes an unusual paternity case analyzed by the AmpF/STR Identifiler Kit, in which father and daughter shared three rare alleles and a mismatch due to a mutation at one microsatellite.
Abstract
Microsatellites exhibit individual genetic differences and are thus extensively used for genetic mapping, personal identification, and paternity testing among others. The AmpF/STR Identifiler Kit is one of the most frequently used coamplification kits for paternity testing and amplifies 15 microsatellites and the Amelogenin locus. In the case at hand, DNA was amplified with the AmpF/STR Identifiler Kit on a GenAmp System 9700 thermal cycler in two separate laboratories. Capillary electrophoresis was carried out on an ABI Prism 310 Genetic Analyzer. The sequencing of rare alleles was performed on fragments eluted from gel by the DNA Gel Extraction Kit. Data were analyzed with AB Software Sequencing Analysis 3.0 and sequences were aligned by Sequence Navigator Software 1.0.1. Data showed that father and daughter shared three rare alleles for D19S433, D18S51, and TH01 microsatellites. The case also revealed a mismatch at the D3S1358 microsatellite. Considering that compatibility had been established for all the loci examined except this one, the author hypothesized a mutational event. The sequencing analysis revealed molecular variations in the regions flanking the motif repeats. The overall results were strongly indicative of kinship and even stronger indications for paternity were derived when the locus in question was excluded from the calculus. Tables, figures, references