NCJ Number
231267
Journal
Journal of Forensic Identification Volume: 60 Issue: 4 Dated: July/August 2010 Pages: 430-438
Date Published
July 2010
Length
9 pages
Annotation
This study examined the use of ultrasonic technology to extract trace amounts of DNA from bones.
Abstract
The present study involves the use of ultrasonic technology to rapidly and effectively extract trace amounts of DNA from bones. The method not only extracts DNA but also preserves its integrity because there is no heating or foaming during the automated extraction process. Additionally, because the system is self contained, there is minimal risk of contamination. This technique allows for the extraction of DNA from bones using acoustic energy which can be manipulated using two different parameters (duty cycle and amplitude). Using pig bones, different acoustic settings were tested to determine which combination produces the highest yield of amplifiable DNA after the entire extraction procedure has been completed. Pig and human DNA were successfully extracted from bones and amplified using this procedure. The results demonstrate the ability to obtain DNA from bone samples using acoustic energy. References, tables, and figures (Published Abstract)