NCJ Number
234615
Journal
Forensic Science International: Genetics Volume: 5 Issue: 3 Dated: June 2011 Pages: 210-215
Date Published
June 2011
Length
6 pages
Annotation
This study explored the ability to obtain DNA profiles from four immunochromatographic test devices that had already yielded positive results with body fluids using LCN methodology.
Abstract
The tests conducted in this study were able to obtain autosomal and Y-STR DNA profiles from most of the immunochromatographic cards which were stored at room temperature for up to 3 months, and DNA profile was obtained from all four types of the cards. The DNA profiles obtained in the study were in agreement with the profiles that were obtained from the donors' reference samples. The intent of the study was to determine the ability of various immunochromatographic devices that are used to confirm the presence of blood and saliva to also be subsequently used as a source for DNA after they have identified the nature of the body fluid. Four types of immunochromatographic cards were used in the study, three for the identification of human blood and one for the identification of human saliva. After the cards were used to identify the fluids of 14 volunteers, they were stored at room temperature for varying lengths of time. Low copy number (LCN) methodology was then used to extract DNA from the used cards. The DNA was then amplified and compared to the DNA reference samples submitted by the volunteers prior to the tests. The results of the test indicate the potential for generating DNA profiles from immunochromatographic testing devices. Figures and references