Developmental Validation of RSIDtm-Saliva: A Lateral Flow Immunochromatographic Strip Test for the Forensic Detection of Saliva

• NCJ Number: 227710
• Journal: Journal of Forensic Sciences Volume: 54 Issue: 4 Dated: July 2009 Pages: 866-873
• Author(s): Jennifer B. Old Ph.D.; Brett A. Schweers Ph.D.; Pravat W. Boonlayangoor Ph.D.; Karl A. Reich Ph.D.
• Date Published: July 2009
• Length: 8 pages
• Publication Format: Article
• Publication Type: Test/Measurement; Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

This paper reports on the developmental validation and testing of the Rapid Stain Identification Test for Saliva (RSIDtm-Saliva), a lateral flow immunochromatographic strip test designed to detect the presence of human salivary alpha-amylase, an enzyme found at high levels in human saliva.

Abstract

The testing demonstrated that RSIDtm-Saliva is accurate, reproducible, and highly sensitive for human saliva. The procedure detected less than 1 ml of saliva. The sensitivity of RSIDtm-Saliva allows investigators to sample a fraction of a questioned stain while retaining the majority for DNA-STR analysis. The RSIDtm-Saliva identified saliva from a variety of materials, including cans, bottles, envelopes, and cigarette butts; and it did not cross-react with blood, semen, urine, or vaginal fluid. The descriptions of materials and methods address the sources of the materials used in RSIDtm-Saliva buffer components, the configuration of the salivary amylase lateral flow test, quantification of the salivary amylase lateral flow tests, specimens used in the testing, the preparation of body fluid extracts, the preparation of mock casework extracts, and DNA extraction and STR DNA analysis. 6 figures and 9 references