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Use of Crossover Immunoelectrophoresis to Detect Human Blood Protein in Soil from an Ambush Scene in Kosovo

NCJ Number
241211
Journal
Journal of Forensic Sciences Volume: 57 Issue: 4 Dated: July 2012 Pages: 873-879
Author(s)
Hugh Tuller, M.A.; Rebecca Saunders, Ph.D.
Date Published
July 2012
Length
7 pages
Annotation
This study examined the survivability of human blood proteins in soils from a year and a half old ambush scene in Kosovo.
Abstract
This study examines the survivability of human blood proteins in soils from a year and a half old ambush scene in Kosovo. A total of 72 soil samples were collected, a number of which were directly associated with bone fragments or bullet projectiles. The samples were examined using crossover immunoelectrophoresis (CIEP) to determine the presence of blood protein and species affiliation. Human blood proteins were identified in 44 of the 72 samples (61 percent) with the majority of the positive observations (29 of 44) found 0.0-4.5 cm below ground surface (65 percent). Chi-squared and two-sample difference of proportions tests confirmed significant differences between samples with and without associated physical evidence and the presence and depth of human blood proteins. While DNA has largely replaced immunological analysis in forensic analyses, our results suggest that in particular situations, CIEP may still be a valuable tool in criminology. Abstract published by arrangement with John Wiley & Sons.

Publication Format
Article
Publication Type
Research (Applied/Empirical)
Language
English
Country
United States of America