NCJ Number
246205
Journal
Journal of Forensic Sciences Volume: 58 Issue: 5 Dated: September 2013 Pages: 1322-1329
Date Published
September 2013
Length
8 pages
Annotation
Simple, reliable, sensitive, and accurate spectrofluorimetric and spectrophotometric methods were proposed for the determination of two selected analgesic drugs, namely tramadol and morphine, in pharmaceuticals and biological fluids.
Abstract
Simple, reliable, sensitive, and accurate spectrofluorimetric and spectrophotometric methods were proposed for the determination of two selected analgesic drugs, namely tramadol and morphine, in pharmaceuticals and biological fluids. The proposed methods were based on the oxidation of the studied drugs by Cerium Ce IV in an acidic medium. The spectrofluorimetric method is based on measuring the relative fluorescence intensity of Ce III arising from Ce IV at 350 nm with an excitation wavelength at 250 nm. The spectrophotometric method involves on addition of a known excess of Ce IV to the studied drugs in an acid medium, followed by the determination of residual Ce IV by reacting with a fixed amount of methyl orange and measuring the absorbance at 510 nm. Different variables affecting the reaction conditions, such as the concentrations of Ce IV, type and concentration of the acid medium, reaction time, temperature, and the diluting solvents, were carefully studied and optimized. Abstract published by arrangement with Wiley.