Preliminary Study on Diffuse Axonal Injury by Fourier Transform Infrared Spectroscopy Histopathology Imaging

• NCJ Number: 246566
• Journal: Journal of Forensic Sciences Volume: 59 Issue: 1 Dated: January 2014 Pages: 231-235
• Author(s): Tiantong Yang M.D.; Guanglong He M.D.; Xiang Zhang M.D.; Lin Chang M.D.; Haidong Zhang M.D.; Mary G. Ripple M.D.; David R. Fowler M.D.; Ling Li M.D.
• Date Published: January 2014
• Length: 5 pages
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

The objective of this study was to evaluate the application of Fourier transform infrared FTIR spectroscopy for detecting diffuse axonal injury DAI in a mouse model.

Abstract

The objective of this study was to evaluate the application of Fourier transform infrared FTIR spectroscopy for detecting diffuse axonal injury DAI in a mouse model. Brain tissues from DAI mouse model were prepared with H&E, silver, and -amyloid precursor protein -APP immunohistochemistry stains and were also studied with FTIR. The infrared spectrum images showed high absorption of amide II in the subcortical white matter of the experimental mouse brain, while there was no obvious expression of amide II in the control mouse brain. The areas with high absorption of amide II were in the same distribution as the DAI region confirmed by the silver and -APP studies. The result suggests that high absorption of amide II correlates with axonal injury. The use of FTIR imaging allows the biochemical changes associated with DAI pathologies to be detected in the tissues, thus providing an important adjunct method to the current conventional pathological diagnostic techniques.