U.S. flag

An official website of the United States government, Department of Justice.

NCJRS Virtual Library

The Virtual Library houses over 235,000 criminal justice resources, including all known OJP works.
Click here to search the NCJRS Virtual Library

Performance of Two 17 Locus Forensic Identification STR Kits-Applied Biosystems's AmpFlSTR NGMSElect and Promega's PowerPlex ESI17 Kits

NCJ Number
Forensic Science International: Genetics Volume: 6 Issue: 5 Dated: September 2012 Pages: 523-531
Torben Tvedebrink; Helle Smidt Mogensen; Maria Charlotte Stene; Niels Morling
Date Published
September 2012
9 pages
This study compared the performance of two recently released 17 loci STR multiplexes for human identification: Applied Biosystems's AmpFlSTR NGMSElect and Promega's PowerPlex ES117.
The comparative parameters were chosen by their relevance in forensic identification and particularly in crime cases. The comparative analyses encompass amplification ability, heterozygote balance, allelic drop-out, drop-in, stutter analysis, and inter-locus balance. Four DNA profiles were analyzed in various concentrations in a serial dilution experiment. The amounts of DNA in the PCR ranged from 3 pg to 420 pg and were analyzed in triplicate using 20.29 and 30 PCR cycles. In order to compare the kits, aliquots from each sample were analyzed with both kits under identical conditions. Furthermore, DNA profiles from 200 reference profiles were analyzed using both kits. The results from the statistical analyses did not indicate any substantial differences of practical relevance between the kits for forensic case work. For all parameters included in this comparative study, the two kits showed no departure from previously observed patterns relative to e.g. the amounts of DNA or amplicon lengths. Based on the analyses, both kits are considered applicable for forensic crime case work. (Published Abstract)