NCJ Number
226991
Journal
Forensic Science International: Genetics Volume: 3 Issue: 2 Dated: March 2009 Pages: 74-79
Date Published
March 2009
Length
6 pages
Annotation
This report describes two novel systems of molecular sex identification suitable for skeletal material using the amelogenin gene, beginning in nitron 2-3, spanning exon 3, and ending in intron 3-4.
Abstract
These new techniques were found to be optimal for sex identification, with both inherent control of isolating many sex-specific features and combined with the use of sensitive micro-fluidic electrophoresis. Using both systems of sex identification, 46.66 percent of specimens produced results. Method one produced 14.28 percent results, and system two produced 57.14 percent results. The procedures were applied to a collection of miscellaneous archaeological skeletons sourced from the Raymond Dart Collection of Human Skeletons. In system one, the primers for the second round of PCR were forward primer AMEL-se and reverse primer AMEL-as. The molecular weight of the final PCR produced was 199 bp. The PCR produced was run on a 1.5 percent agarose gel stained with actinium bromide. The PCR produced was then purified using a Qiagen QIAquick purification Kit. Under system two, the primers used in the second round of PCR were forward primer AMEL-fi and reverse primer AMEL-ri. The molecular weight for the X was 188 bp and for the Y was 194 bp. The PCR product for each sample was analyzed using the Bio-Rad Experion DNA 1 K system for indel analysis. For the optimization of these methods, six human blood and two human bone controls were used. The materials and methods were designed using guidelines for aDNA research. 4 figures, 3 tables, and 33 references