Microanalysis of Carbon Monoxide in Blood by Head-Space Capillary Gas Chromatography

• NCJ Number: 148154
• Journal: Journal of Forensic Sciences Volume: 39 Issue: 2 Dated: (March 1994) Pages: 473-478
• Author(s): J Van Dam; P Daenens
• Date Published: 1994
• Length: 6 pages
• Publication Format: Article
• Publication Type: Report (Technical Assistance)
• Language: English
• Country: United States of America

Annotation

A gas chromatographic procedure for the determination of carbon monoxide in blood was improved by using a capillary system in combination with a micro thermal conductivity detector.

Abstract

The technique involved the use of a capillary Molecular Sieve column in combination with a Micro Thermal Conductivity Detector. This system is very sensitive with high resolutions. The analytical time is reduced to approximately 2 minutes. This amounts to a reduction of the analytical time by a factor of two to four in comparison with methods described by others. In addition to a rapid analysis, it is also possible to reduce the liberation time of 40 minutes. Using sulfuric acid as the liberating agent, a gradual and reproducible release of carbon monoxide was seen. This approach shortens the time required in emergency cases to prepare a sample. Figures and 5 references (Author abstract modified)