NCJ Number
134483
Journal
Canadian Society of Forensic Science Journal Volume: 24 Issue: 3 Dated: (September 1991) Pages: 147-164
Date Published
1991
Length
18 pages
Annotation
Infrared and mass spectra data are presented for testosterone and 11 of its 14 beta esters as methods of analysis and published reference spectra for anabolic steroids in pharmaceutical preparations.
Abstract
Infrared spectrometer (IR) conditions used for the analysis included one/centimeter point-to-point resolution as nujol mulls on sodium chloride disks. Suspensions of testosterone were extracted with chloroform, and the extract evaporated for IR or diluted to 0.2 milligram(mg)/milliliter(ml) for gas chromatography (GC). The testosterone esters were dissolved in vegetable oil, extracted with methanol, and at the same concentrations were injected into a capillary column GC or into a gas chromatograph(GC)-mass spectroscopy. The GC column temperature program was 250 degrees Centigrade for 2 minutes, 10 degrees/minute to 300 degrees, then held at 300 for 8 minutes. The injector and detector temperatures were 315 Centigrade. The carrier gas was helium with a flow rate of 1 ml/minute (min). The IR spectra of testosterone and the 11 esters were distinguishable from each other. Testosterone exhibited the ketone band at 1670/centimeter (cm), while the esters exhibited the ketone ban from 1660 to 1670/cm and the ester ban at 1735 to 1750/cm. Under these GC conditions, testosterone and the 11 esters exhibit baseline separation with the exception of testosterone undecanoate and testosterone undecanoate which appear as a merged peak. 27 figures and 11 references