NCJ Number
172016
Journal
Journal of Forensic Sciences Volume: 42 Issue: 5 Dated: (September 1997) Pages: 907-910
Date Published
1997
Length
4 pages
Annotation
This study tested the forensic usefulness of the HumCD4 short tandem repeat (STR) system.
Abstract
The short tandem repeat system HumCD4 was amplified by the polymerase chain reaction (PCR) on blood samples from 304 unrelated Austrian Caucasians and analyzed by horizontal, non-denaturing polyacrylamide electrophoresis. The mean exclusion chance was 0.417, the discriminating power 0.850 and the heterozygosity rate 0.628. The observed phenotype distribution is in Hardy-Weinberg equilibrium. In 100 families (200 meioses) no mutations were found. Sufficient amplification could be achieved with as little as 80 pg of high molecular weight cell-line DNA. In a degradation experiment DNA extracted from bloodstains stored for up to 28 days in a moist chamber and DNA boiled for up to 18 minutes could be amplified. A duplex PCR with TH101 is proposed. CD4 proved satisfactory concerning sensitivity and sufficiently resistant to degradation of DNA. The high electrophoretic resolution of this locus which allows a running time of only 30 minutes is most unique. Although MEC and DP were relatively low, a coamplification with TH01 overcomes this limitation without consuming more template or time. The STR system CD4, therefore, seems to be useful for analyzing biological traces for forensic purposes. Figures, tables, references