NCJ Number
90594
Editor(s)
I S Lurie,
J D Wittwer
Date Published
1983
Length
346 pages
Annotation
This book on high-performance liquid chromatography (HPLC) -- a rapid, accurate, and precise technique for the analytical determination of compounds -- provides a basic understanding of the technique and presents applications relevant to forensic analysis. It is intended for forensic analysts with and without experience in HPLC, as well as for chemists in related disciplines such as pharmaceutical science and clinical chemistry.
Abstract
A chapter on the principles of HPLC considers classic versus modern liquid chromatography, chromatographic modes, basic references, and theoretical considerations, while the discussion of the hardware for HPLC addresses the solvent delivery systems, sample introduction, columns, detectors, and data handling. A chapter is devoted to the use of silica, the most widely used adsorbent in adsorption chromatography. Attention is given to silica packings, moderators, the mobile phase, evaluation of column performance, the care and cleaning of columns, and applications. Since about 80 percent of all HPLC separations are performed using bonded-phase columns in the reversed-phase mode, a chapter recognizes the major importance of this type of chromatography in forensic drug analysis. The use of bonded-phase columns is also described in nondrug analyses. Future developments are considered as well. The discussion of liquid exclusion chromatography, a means of overcoming some of the problems inherent in making a comparative analysis, provides an overview of the process, separation mechanism and solute retention, and applications. Remaining chapters examine the use of HPLC in forensic toxicology and in the analyses of explosives and writing inks. Chapter references are provided along with author and subject indexes.