NCJ Number
212939
Journal
Journal of Forensic Sciences Volume: 51 Issue: 1 Dated: January 2006 Pages: 183-187
Date Published
January 2006
Length
5 pages
Annotation
This paper reports on the genetic polymorphism observed at 15 short tandem repeat loci in 4 aboriginal populations of Bengal, India.
Abstract
The short tandem repeat loci are D3S1358, TH01, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, CSF1PO, vWA, D8S1179, TPOX, D2S1338, D19S433, and FGA. The four tribal groups studied were Australoids. The Karmali tribal group belongs to the traditional blacksmith community. The Kora tribal group is a tribal community from eastern India that consists of earth workers and well diggers. Both of the tribes use an Indo-Aryan language called "Khotta." The Maheli tribe is primarily associated with Indigo cultivation, and members speak the Indoaryan language of Sadri. The Lodha tribe derives its name from a word that means "forest robbers." They use the Austro-Asiatic language called "Lodha." Tribe members are concentrated in the west Midnapore district of Chhotanagpur. They were considered a criminal tribe until the revocation of the Criminal Tribe Act. Blood samples were collected randomly from various rural areas. Genomic DNA was isolated through the organic method of extraction. The quantitation of DNA was conducted with the Quantiblot kit, and PCR amplification of the mentioned short tandem repeat loci was performed with the AmpFlSTR Identifiler Multiplex system. Analysis was performed on a 4 percent polyacrylamide gel with an ABI Prism 377 Automated DNA Sequencer machine. Genotype classification was performed by comparison with allelic ladders provided with the Identifiler kit. 6 tables and 11 references