Evaluation of Variation in Control Region Sequences for Hispanic Individuals in the SWGDAM mtDNA Data Set

• NCJ Number: 214494
• Journal: Journal of Forensic Sciences Volume: 51 Issue: 3 Dated: May 2006 Pages: 566-573
• Author(s): Marc W. Allard Ph.D.; Deborah Polanskey B.S.; Mark R. Wilson Ph.D.; Keith L. Monson Ph.D.; Bruce Budowle Ph.D.
• Date Published: May 2006
• Length: 8 pages
• Sponsoring Agency: US Dept of Energy; US Federal Bureau of Investigation
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

The Hispanic dataset of the Scientific Working Group on DNA Analysis Methods (SWGDAM) was analyzed to determine the diversity, phylogeny, and relevant single nucleotide polymorphisms (SNPs) that describe haplogroup patterns for Hispanic Americans (n=686) and to assess the degree of admixture regarding mitochondrial DNA (mtDNA).

Abstract

The largest component of admixture based on MtDNA analysis came from the four major haplogroups previously observed in Native American ancestry, including A (29.3 percent), B (15.7 percent), C (20.6 percent), and D (4.8 percent). European (17.8 percent) and African (11.8 percent) were also observed within this dataset. The descriptive analyses found that the SWGDAM Hispanic dataset is similar to published Native American and Hispanic datasets. Of the 686 individuals in the dataset, 245 came from Texas or New Mexico, with the remainder coming from Connecticut (n=148), Illinois (N=5), or from an unknown location (n=288). In comparing SWGDAM Hispanic samples from the Southwest with other SWGDAM Hispanic samples, a greater percentage of Native American haplogroups were present (79.9 percent); and there were fewer African-American haplogroups (4.5 percent). A total of 234 SNPs were found in the dataset, including 36 newly reported variable positions. The SNPs ranged from having 1 to 31 changes on the phylogenetic tree, with site 16519 having the most variation. There was an average of 3.9 character changes for each variable position on the tree. The most variable sites (13 or more changes each listed from fastest to slowest) by length (L) were 16519 (L=31), 16189 (L=23), 152 (L=23), 16311 (L=19), 146 (L=17), 195 (L=17), 16093 (L=15), 16362 (L=14), 16129 (L=13), 150 (L=13), and 153 (L=13). A total of 27 SNPs were selected to identify all clusters that contained 1 percent (N=7) or more individuals in the Hispanic data set. The report's description of research methodology addresses data availability, phylogenetic methods, sequence alignment, and the ranking of SNPs. 5 tables and 65 references