Effects of Argon Laser Light, Alternate Source Light, and Cyanoacrylate Fuming on DNA Typing of Human Bloodstains

• NCJ Number: 140928
• Journal: Journal of Forensic Sciences Volume: 38 Issue: 1 Dated: (January 1993) Pages: 184-191
• Author(s): E Shipp; R Roelofs; E Togneri; R Wright; D Atkinson; B Henry
• Date Published: 1993
• Length: 8 pages
• Publication Format: Article
• Publication Type: Report (Technical)
• Language: English
• Country: United States of America

Annotation

Restriction fragment length polymorphism (RFLP) profile results were obtained from deoxyribonucleic acid (DNA) isolated from human bloodstains that had been subjected to cyanoacrylate ester ("superglue") guming, argon ion laser light, and alternate light sources.

Abstract

Bloodstains present on pieces of white cotton cloth were exposed to cyanoacrylate ester and then subjected to one of the three light sources at different wavelengths. Although length of exposure to light sources varied, the light source was always 17.5 centimeters from the treated bloodstain. The potential effect of cyanoacrylate ester on RFLP analysis of DNA was determined by exposure of dried bloodstains to superglue vapors. Bloodstain analysis revealed that an RFLP profile using the polymorphic probe MS1 (locus D1S7) could be generated successfully after exposure of bloodstains to cyanoacrylate ester. This observation has relevance in examining objects for fingerprints which also have blood on their surfaces. 8 references, 6 tables, and 3 figures