NCJ Number
164162
Journal
Japanese Journal of Legal Medicine Volume: 50 Issue: 4 Dated: (August 1996) Pages: 255-257
Date Published
1996
Length
3 pages
Annotation
DNA typing by triplex affinity capture PCR is simple, rapid, and well-suited for paternity testing and population studies of microsatellites.
Abstract
Triplex affinity capture (TAC) is an effective method for isolation of human genomic DNA in recombinant DNA technology. It is based on the formation of triple-helices between purine-rich oligonucleotides and homopyrimidine/homopurine sequences of double-stranded DNA in the presence of Mg2+3. In this procedure, double-stranded target DNA's are captured by a biotinylated oligonucleotide probe that has been bound to streptavidin-coated magnetic beads, and the capture can be released by addition of EDTA for use in PCR. Using this principle, this study extracted DNA for PCR-based DNA typing. The procedure amplified the MCT118, ACTBP2, FGA, D8S320, D11S488, and THO1 loci from the captured DNA. The researchers were able to obtain PCR-ready DNA only from such bloodstains on hard objects as could be scraped off, but not from bloodstains on a cotton cloth. Thus, the TAC approach has a restricted application in bloodstain analysis but is well-suited for paternity testing and population studies of microsatellites. 1 figure and 9 references