NCJ Number
237526
Journal
Forensic Science International: Genetics Volume: 6 Issue: 1 Dated: January 2012 Pages: 108-112
Date Published
January 2012
Length
5 pages
Annotation
This study examined the Applied Biosystems SGM Plus kit being evaluated in the Israel Police DNA database laboratory.
Abstract
During the course of routine database sample analysis in the Israel Police DNA database, an off-ladder D3S1358 allele, calculated to be greater than 22.1, extending into the adjacent vWA locus was observed using Applied Biosystems SGM Plus kit. To verify the size of this D3S1358 long allele and to ensure it was not part of a trialle pattern in the neighboring locus, the sample was amplified using three of the European new generation STR multiplex kits: NGM(TM) (Applied Biosystem), Powerplex ESX and ESI (Promega). The results of these amplifications determined the variant to be a 22 allele. Subsequent sequencing confirmed this designation and revealed a nucleotide polymorphism. Ten additional SGM Plus profiled samples with D3S1358 alleles larger than 19, were re-analyzed using NGM(TM) and Powerplex ESX which also showed discordance in the calculated results between original SGM Plus designations and those obtained with the European new generation multiplexes. (Published Abstract)