Developmental Validation of the PowerPlex Fusion System for Analysis of Casework and Reference Samples: A 24-Locus Multiplex for New Database Standards

• NCJ Number: 248143
• Journal: Forensic Science International Genetics Volume: 12 Dated: September 2014 Pages: 69-76
• Author(s): Kathryn Oostdik; Kristy Lenz; Jeffrey Nye; Kristin Schelling; Donald Yet; Scott Bruski; Joshua Strong; Clint Buchanan; Joel Sutton; Jessica Linner; Nicole Frazier; Hays Young; Learden Matthies; Amber Sage; Jeff Hahn; Regina Wells; Natasha Williams; Monica Price; Jody Koehler; Melisa Staples; Katie L. Swango; Carolyn Hill; Karen Oyerly; Wendy Duke; Lesley Katzilierakis; Martin G. Ensenberger; Jeanne M. Bourdeau; Cynthia J. Spercher; Benjamin Krenke; Douglas R. Storts
• Date Published: September 2014
• Length: 8 pages
• Publication Format: Article
• Language: English
• Country: Ireland

Annotation

This project performed developmental validation to demonstrate the quality and robustness of the PowerPlex Fusion System across a number of variables.

Abstract

Validation results verify that the PowerPlex fusion System is a robust and reliable STR typing multiplex suitable for human identification. Following SWGDAM and NDIS guidelines, 12 forensic and research laboratories showed strong performance throughout validation testing for the Powerplex Fusion System. Simultaneous amplification was achieved for the following loci: Amelogenin, D3S1358, D1S156, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, DSS818, TPOX, DYS391, D8S1179, D12S391, D19S433, FGA, and D22S1045. The comprehensive list of loci amplified by the system generates a profile compatible with databases based on either the expanded CODIS or European Standard Set (ESS) requirements. Twelve forensic and research laboratories demonstrated strong performance throughout validation testing for the PowerPlex Fusion System. Minimal cross-reactivity, low-level sensitivity and mixture detection, precise and accurate allele calls, and robust performance with casework samples and in the presence of inhibitors were observed. Strong amplification and minimal artifacts were produced under several suboptimal PCR conditions. Multiple DNA sources encompassing extracted DNA, FTA card punches, swabs, and nonFTA punches achieved full and concordant profiles. Materials and methods are described. 6 figures, 1 table, and 16 references