U.S. flag

An official website of the United States government, Department of Justice.

NCJRS Virtual Library

The Virtual Library houses over 235,000 criminal justice resources, including all known OJP works.
Click here to search the NCJRS Virtual Library

Developmental Validation of the PowerPlex 18D System, a Rapid STR Multiplex for Analysis of Reference Samples

NCJ Number
Forensic Science International: Genetics Volume: 7 Issue: 1 Dated: January 2013 Pages: 129-135
Kathryn Oostdik; Julie French; Donald Yet; Briana Smalling; Craig Nolde; Peter M. Vallone; Erica L.R. Butts; Carolyn R. Hill; Margaret C. Kline; Theresa Rinta; Amy M. Gerow; Stacey R. Allen; Christopher K. Huber; John Teske; Benjamin Krenke; Martin Ensenberger; Patricia Fulmer; Cynthia Sprecher
Date Published
January 2013
7 pages
In order to determine the suitability of the PowerPlex 18D System for human identification testing, this project assessed it for sensitivity, concordance, inhibitor tolerance, and performance with thermal cycling and reaction condition variation, following SWGDAM developmental validation guidelines.
The results demonstrated that the PowerPlex 18D System can be used for forensic and human identification testing. Since short tandem repeat (STR) markers remain the foundation of human identification throughout the world, new STR multiplexes require rigorous testing to ensure the assays are sufficiently robust and reliable for genotyping purposes. The PowerPlex 18D System was created for the direct amplification of buccal and blood samples from FTA storage cards and reliably accommodates other sample materials. The PowerPlex 18D System allows simultaneous amplification of the 13 CODIS loci and amelogenin, along with four additional loci: Penta E, Penta D, D2S1338, and D19S433. 4 tables, 4 figures, and 23 references