NCJ Number
223872
Journal
Journal of Forensic Sciences Volume: 53 Issue: 4 Dated: July 2008 Pages: 838-852
Date Published
July 2008
Length
15 pages
Annotation
This paper reports on the development and validation of the AmpFlSTR MiniFiler PCR Amplification Kit, which was designed to amplify as miniSTRs eight of the largest sized loci in the AmpFlSTR Identifiler PCR Amplifaction Kit, which, together with the gender-identification locus Amelogenin, enables simultaneous amplification of the loci that often fail detection during the amplification of compromised DNA samples.
Abstract
The results from the models of PCR degradation and inhibition and nonprobative samples show that the MiniFiler kit can be useful in amplifying DNA under conditions in which other commercial autonomic STR kits yield partial or no profiles. This kit is thus a useful and robust complement to conventional STR kits and will be especially applicable to challenging situations that involve the identification of human remains. The authors recommend that each laboratory conduct its own internal validation according to FBI National Standards and SWGDAM guidelines. The eight miniSTRs are D7S820, D13S317, D16S539, D21S11, D2S1338, D18S51, CSF1PO, and FGA). Five of these STR loci also are some of the largest loci in the AmpFlSTR SCM Plus kit: D16S539, D21S11, D2S1338, D18S51, and FGA. The description of materials and methods addresses the DNA samples; primer set optimization; PCR components; thermal cycling parameters; accuracy, prevision, and stutter studies; PCR amplification; sample electrophoresis and data analysis; species specificity; sensitivity study; models of DNA degradation and PCR inhibition; nonprobative samples; mixture studies; and statistical analysis. 7 tables, 10 figures, and 33 references