Application of a Solid-Phase Microextraction Technique for the Detection of Urinary Methamphetamine and Amphetamine by Gas Chromatography

• NCJ Number: 166785
• Journal: Canadian Society of Forensic Science Volume: 29 Issue: 2 Dated: (June 1996) Pages: 43-48
• Author(s): K Ameno; C Fuke; S Ameno; H Kinoshita; I Ijiri
• Date Published: 1996
• Length: 6 pages
• Publication Format: Article
• Publication Type: Report (Technical Assistance)
• Language: French; English
• Country: Canada

Annotation

This paper describes a method for the simple and rapid detection of methamphetamine and amphetamine simultaneously in urine, based on gas chromatography with a solid-phase micro-extraction technique.

Abstract

The research used an SPME filter with a fiber holder for manual use. After the addition of urine, NaCl, and 1-phenylbutylamine, the sample was adjusted to pH12 with NaOH(10N) and then rapidly sealed with a teflon-coated septum. After immersion of the SPME fiber in the sample for 20 minutes and then washing promptly in a bugger, the SPME needle was inserted into the injection port of the gas chromatographer and the fiber was extruded. Each peal of methamphetamine, amphetamine, and 1-phenylbutylamine was clearly separated. The linearity of the amphetamine and methamphetamine were in the ranges of 0.25-10 and 0.1-1.0 micrograms per milliliter, respectively. This SPME technique is applicable without using organic solvents, as well as simple, rapid, and useful for the practical detection of methamphetamine and amphetamine in urine. Figures and 12 references (Author abstract modified)