21-Locus Autosomal SNP Multiplex and its Application in Forensic Science

• NCJ Number: 246538
• Journal: Journal of Forensic Sciences Volume: 59 Issue: 1 Dated: January 2014 Pages: 5-14
• Author(s): Guangwei Hou Ph.D.; Xianhua Jiang M.D.; Yanyan Yang M.D.; Fei Jia M.D.; Qiang Li M.D.; Jinling Zhao M.D.; Fei Guo M.D.; Limin Liu M.D.
• Date Published: January 2014
• Length: 10 pages
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

To develop a cost-effective technique for single-nucleotide polymorphism SNP genotyping and improve the efficiency to analyze degraded DNA, we have established a novel multiplex system including 21-locus autosomal SNPs and amelogenin locus, which was based on allele-specific amplification ASA and universal reporter primers URP.

Abstract

To develop a cost-effective technique for single-nucleotide polymorphism SNP genotyping and improve the efficiency to analyze degraded DNA, we have established a novel multiplex system including 21-locus autosomal SNPs and amelogenin locus, which was based on allele-specific amplification ASA and universal reporter primers URP. The target amplicons for each of the 21 SNPs arranged from 63 base pair bp to 192 bp. The system was tested in 539 samples from three ethnic groups Han, Mongolian, and Zhuang population in China, and the total power of discrimination TPD and cumulative probability of exclusion CPE were more than 0.99999999 and 0.98, respectively. The system was further validated with forensic samples and full profiles could be achieved from degraded DNA and 63 case-type samples. In summary, the multiplex system offers an effective technique for individual identification of forensic samples and is much more efficient in the analysis of degraded DNA compared with standard STR typing. Abstract published by arrangement with Wiley.