This study introduces a rotationally-driven dynamic solid phase sodium bisulfite conversion disc for forensic epigenetic sample preparation.
Epigenetic analysis requires sodium bisulfite conversion (BSC), a sample preparation method that is time-consuming, labor-intensive, prone to contamination, and characterized by DNA loss and fragmentation. To provide an alternative method for BSC that is more amenable to integration with the forensic DNA workflow, the authors of this study describe a rotationally-driven, microfluidic method for dynamic solid phase-BSC (dSP-BSC) that streamlines the sample preparation process in an automated format, capable of preparing up to four samples in parallel. The method permitted decreased incubation intervals by ∼36% and was assessed for relative DNA recovery and conversion efficiency and compared to gold-standard and enzymatic approaches. (Published Abstract Provided)
Downloads
Similar Publications
- Introducing the NIJ Forensic Intelligence Framework: Pillars and Guiding Principles for Successful Implementation
- Firearm Purchase Behavior and Subsequent Adverse Events
- Recovery and Detection of Ignitable Liquid Residues from the Substrates by Solid Phase Microextraction – Direct Analysis in Real Time Mass Spectrometry