This article shows the utility of diode array ultraviolet (UV) detection for aiding in the identification of synthetic cathinones, including different sub-classes and positional isomers.
For 35 synthetic cathinones, unique UV spectra were obtained for seven sub-classes, including mostly beta ketones, where position and type of substitution on benzene rings give rise to differences in UV maxima and relative intensity of the spectral bands. This aspect is key to distinguishing positional isomers that contain differences in R substitution (mono and di) around the benzene ring, which provides complementary information to electron ionization mass spectrometry, where the latter technique cannot distinguish between these types of positional isomers. In addition, it is possible to ascertain the substitution position based on the UV spectra. For ten sets of positional isomers, it was possible to distinguish most of the positional isomers within a set. For ultra-high performance super critical fluid chromatography (UHPSFC) versus reversed phase ultra-high performance liquid chromatography (UHPLC), there was at least a 10 nm blue shift in UV maximum (shift to shorter wavelengths). This highlights the importance of considering the effect of mobile phase on the UV maximum when performing method development in UHPSFC. (publisher abstract modified)
Downloads
Similar Publications
- The Collection, Preservation, and Processing of DNA Samples from Decomposing Human Remains for More Direct Disaster Victim Identification (DVI)
- Audit of the Office of Justice Programs Drug and Mental Health Treatment Grants Awarded to My Health My Resources of Tarrant County, Fort Worth, Texas
- Examining the Relationship between Aptamer Complexity and Molecular Discrimination of a Low-Epitope Target