Performance Evaluation and Optimization of Multiplex PCRs for the Highly Discriminating OSU 10-Locus Set Y-STRs

• NCJ Number: 239615
• Journal: Journal of Forensic Sciences Volume: 57 Issue: 1 Dated: January 2012 Pages: 52-59
• Author(s): Erin Hanson, Ph.D.; Julie L. Maybruck, Ph.D.; Jack Ballantyne, Ph.D.; Paul A. Fuerst, Ph.D.
• Date Published: January 2012
• Length: 8 pages
• Grant Number(s): 2005-MU-MU-K044
• Agencies: NIJ-Sponsored
• Publication Type: Report (Study/Research)

Annotation

This study evaluated the OSU 10-locus set to test the effectiveness of the markers on forensic samples.

Abstract

In a previous study, a new set of Y-chromosome short tandem repeats, the OSU 10-locus set (MPM1 and MPM2), was shown to have a higher discrimination power when evaluated against the 10 SWGDAM loci on a common population panel. Here, we describe the optimization of the multiplex reactions using dye-labeled primers followed by performance evaluations. The loci exhibited high precision, human male specificity, reliability in different body fluids, high sensitivity, stability, and the ability to amplify nonprobative casework and mixture samples. Stutter for the all of the loci, with the exception of the highly polymorphic locus DYS688, was similar to that observed for autosomal loci. The results of the performance evaluations reinforced the utility of these loci. Published by arrangement with John Wiley & Sons.