This study used a fully continuous probabilistic genotyping software to compare the effectiveness of amplifying a single sample compared to splitting the sample and conducting a joint analysis of replicate amplifications
Previous studies that have examined whether splitting the DNA extract for replicate amplification versus maximizing the template available for a ‘one-shot’ amplification either examined the benefits of using replicates (without a comparison to a single amplification) or used semi-continuous probabilistic software that ignores peak height information. The current study shows that the one-shot approach is marginally better than splitting the DNA extract across a range of contributor numbers and template amounts. Where there is unexpected peak height variability or drop-in within the profile not modelled during interpretation, a replicate approach may be better. (publisher abstract modified)
Downloads
Related Datasets
Similar Publications
- Modeling Bone Surface Morphology: A Fully Quantitative Method for Age-at-Death Estimation Using the Pubic Symphysis
- Accurate and rapid electrochemical detection of fentanyl in hydrophobic ionic liquid at subzero temperatures
- Experimental and numerical investigations for the prediction of depth of calcination of gypsum plasterboards under fire exposure