Previous studies have shown that nylon flocked swabs outperform traditional fiber swabs in DNA recovery due to their innovative design and lack of internal absorbent core to entrap cellular materials. The microFLOQ Direct swab, a miniaturized version of the 4N6 FLOQSwab, has a small swab head that is treated with a lysing agent which allows for direct amplification and DNA profiling from sample collection to final result in less than 2 hours. Additionally, the microFLOQ system subsamples only a minute portion of a stain and preserves the majority of the sample for subsequent testing or re-analysis, if desired. The current study made comparisons to traditional methods in assessing the robustness of this alternate workflow. Controlled studies with 1:19 and 1:99 dilutions of bloodstains and saliva stains consistently yielded higher STR peak heights than standard methods with 1 ng input DNA from the same samples. Touch samples from common items yielded single source and mixed profiles that were consistent with primary users of the objects. With this novel methodology/workflow, no sample loss occurs; therefore, more template DNA is available during amplification. This approach may have important implications for analysis of low quantity and/or degraded samples that plague forensic casework. (publisher abstract modified)
Downloads
Similar Publications
- Harmonizing the Forensic Nomenclature for STR Loci D6S474 and DYS612
- DNA Contamination, Degradation, Damage and Associated Microbiomes: A Comparative Analysis through Massive Parallel Sequencing and Electrophoresis
- Theoretical Insights into the Gas-Phase Oxidation of 3-Methyl-2-butene-1-thiol by the OH Radical: Thermochemical and Kinetic Analysis