The project succeeded in showing that NGS coupled with a probe capture enrichment system can be used for deep sequencing the entire mitochondrial genome and nSNP markers. This procedure can be applied to both nuclear and mtDNA analysis of degraded samples, including bone specimens, and limited DNA amounts (1-100 pg). Thus, these assays can significantly improve discriminatory power compared to current mitochondrial HVI/HVII sequences analysis; and they increase the success rate of genotyping degraded and limited samples. 3 figures and listings of names and descriptions of protocols, probe capture panel, and software programs
Downloads
Similar Publications
- An Affinity-Free, Centrifugal Microfluidic System for Rapid, Automated Differential Extraction
- Understanding the Broader Impacts of Body Worn Cameras on Police Work and Community Perceptions: A Multi-Method Assessment
- Experimental and Numerical Investigation of Gypsum Calcination under Fire Exposure