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Advancing mitochondrial genome data interpretation in missing persons casework

NCJ Number
302728
Journal
Forensic Science International Genetics Supplement Series Volume: 7 Issue: 1 Dated: 2019 Pages: 721-723
Author(s)
Date Published
December 2019
Length
3 pages
Annotation

Since massively parallel sequencing techniques have made possible the adoption of mitogenome sequencing for missing persons casework, the current project expanded the family pedigree and population data necessary for sequence comparisons and statistical analysis. 

Abstract

Complete mitochondrial genome (mitogenome) haplotypes were generated by the Armed Forces Medical Examiner System’s Armed Forces DNA Identification Laboratory, using an automated laboratory workflow and robust analysis pipeline. Over 600 CEPH family pedigree samples from 87 lineages were processed in order to characterize the expected number of differences in mitogenome sequences between maternal relatives. Although stochastic artifacts in the CEPH cell lines elevated the point heteroplasmy rate three-fold compared to population sample data (1.8 vs. 0.46-point heteroplasmies per individual, on average), the CEPH family sequences exhibited a maximum of two substitutions between any two maternal relatives in nearly 500 generational events. The family study furthermore showed that elevating the variant detection threshold may create false substitutions at heteroplasmic sites. Results from an initial 2000 reference database samples indicate a first-pass success rate of 85 percent, with 97 percent unique mitogenome haplotypes. These data demonstrate advancements toward the interpretation of mitogenome sequence data in missing persons casework. (publisher abstract modified)

Date Published: December 1, 2019