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Specificity, Sensitivity, and Operability of RSID-Urine for Forensic Identification of Urine: Comparison with ELISA for Tamm-Horsfall Protein

NCJ Number
242522
Journal
Journal of Forensic Sciences Volume: 57 Issue: 6 Dated: November 2012 Pages: 1570-1573
Author(s)
Tomoko Akutsu, Ph.D.; Ken Watanabe, Ph.D.; Koichi Sakurada, D.D.S., Ph.D.
Date Published
November 2012
Length
4 pages
Annotation
In this study, the specificity, sensitivity, and operability of RSID-Urine, a new immunochromatographic test for urine identification, was evaluated and compared with ELISA detection of Tamm-Horsfall protein (THP).
Abstract
In this study, the specificity, sensitivity, and operability of RSID-Urine, a new immunochromatographic test for urine identification, was evaluated and compared with ELISA detection of Tamm-Horsfall protein (THP). Urine was successfully identified among other body fluids using RSID-Urine and ELISA detection of THP. The detection limit of RSID-Urine equated to 0.5 uL of urine; although the sensitivity of RSID-Urine may be lower than that of ELISA detection of THP, it is thought to be sufficient for application to casework samples. However, results from RSID-Urine must be interpreted with caution when the sample may have been contaminated with blood or vaginal fluid, because this might inhibit urine detection. The RSID-Urine assay can be performed in just 15 min by dropping the extracted sample onto the test cassette. Therefore, RSID-Urine should be an effective tool for the forensic identification of urine, in addition to ELISA detection of THP. Abstract published by arrangement with John Wiley & Sons.