Simultaneous Gas Chromatography/Mass Spectrometry Assay of Methadone and 2-Ethyl-1, 5-Dimethyl-3, 3-Diphenylpyrrolidine (EDDP) in Urine

• NCJ Number: 128917
• Journal: Journal of Forensic Sciences Volume: 36 Issue: 2 Dated: (March 1991) Pages: 548-555
• Author(s): L D Baugh; R H Liu; A S Walia
• Date Published: 1991
• Length: 8 pages
• Publication Format: Article
• Publication Type: Research (Applied/Empirical)
• Language: English
• Country: United States of America

Annotation

An efficient extraction and gas chromatography/mass spectrometry (GC/MS) procedure has been developed for the simultaneous determination of methadone and 2-ethyl-1, 5-dimethyl-3, 3-diphenylpyrrolidine (EDDP) in urine samples.

Abstract

The merits of this procedure include: (1) effective high-volume sample processing; (2) excellent gas chromatography characteristics; (3) high precision for quantitative methadone determination -- 1.0 percent coefficient of variation (CV) for GC/MS injection replicates and 1.2 percent for extraction replicates; (4) excellent linearity within the range (0 to 1200 ng/mL) studied; and (5) adequate detection limits (50 ng/mL) for most practical purposes. The detection limit for methadone may be improved 40-fold by using a different internal standard. 6 figures and 5 references (Author abstract)