Simple Identification Method of Saliva by Detecting Streptococcus Salivarius Using Loop-Mediated Isothermal Amplification

• NCJ Number: 233512
• Journal: Journal of Forensic Sciences Volume: 56 Issue: S1 Dated: January 2011 Pages: S158-S161
• Author(s): Hiroaki Nakanishi, Ph.D.; Takeshi Ohmori, Ph.D.; Masaaki Hara, Ph.D.; Aya Takada, M.D.; Hideki Shojo, Ph.D.; Noboru Adachi, M.D.; Kazuyuki Saito, M.D.
• Date Published: January 2011
• Length: 4 pages
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

The authors previously reported that detection of Streptococcus salivarius is feasible for proving the presence of saliva in a forensic sample. In the current study, a simple and rapid method for the detection of S. salivarius in forensic samples was developed that uses loop-mediated isothermal amplification (LAMP).

Abstract

The LAMP primer set was designed using S. salivarius-specific sequences of glucosyltransferase K. To simplify the procedure, the sample was prepared by boiling and mutanolysin treatment only, and the entire analytical process was completed within 2.5 h. The cut-off value was set at 0.1 absorbance units, measured at 660 nm, upon termination of the reaction. S. salivarius was identified in all saliva samples, but was not detected in other body fluids or on the skin surface. Using this method, S. salivarius was successfully detected in various mock forensic samples. The authors therefore suggest that this approach is useful for the identification of saliva in forensic practice. (Published Abstract)