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Sensitive Identification of Hemoglobin in Bloodstains From Different Species by High Performance Liquid Chromatography With Combined UV and Fluorescence Detection

NCJ Number
150629
Journal
Journal of Forensic Sciences Volume: 39 Issue: 4 Dated: (July 1994) Pages: 1018-1025
Author(s)
J Andrasko; B Rosen
Date Published
1994
Length
8 pages
Annotation
High performance liquid chromatography (HPLC) was used for the identification of the species involved in bloodstains from some common household and wild animals from Sweden; the use of a combination of ultraviolet (UV) and fluorescence detection improved the sensitivity of the method and permitted a better differentiation of human and animal bloodstains.
Abstract
Bloodstains from humans as well as animals often encountered in forensic casework were analyzed using HPLC with reversed-phase large-pore columns. This technique could distinguish bloodstains from common wild and domestic animals with one exception: dog and wolf. The use of fluorescence detection increased the sensitivity of the method. The method was also able to identify human bloodstains in amounts down to 1-2 nanoliters of blood. The method has the advantage of simplicity, speed, and sensitivity for use in forensic practice. Figures, table, and 7 references (Author abstract modified)