This paper explores the use of rolling circle amplification (RCA) in DNA typing systems.
Rolling circle amplification (RCA) is a robust enzymatic process in which a circular DNA molecule serves as a template for exponential amplification of molecular targets of interest. Random primers that have bound to DNA sequences across the circularized DNA template initiate copying via the action of a highly processive DNA polymerase. Following a cascade of strand displacement events, long tandem copies of the original template sequence are generated. Because the template molecule is in a circular format, it essentially serves as an infinite linear template. In conjunction with selective enrichment techniques, RCA may prove highly useful in the selectivity and sensitivity of current DNA typing systems. In addition, circularization potentially may allow for amplification of fragmented and low copy number DNA encountered in forensic casework samples that can be subsequently typed. (Published Abstract Provided)