This review explains the pyrosequencing workflow and illustrates the step-by-step chemistry, followed by a description of the assay design and factors to keep in mind for an exemplary assay.
The recent development of small, single-amplicon-based benchtop systems for pyrosequencing has opened up a host of novel procedures for applications in forensic science. Pyrosequencing is a sequencing by synthesis technique, based on chemiluminescent inorganic pyrophosphate detection. Existing and potential forensic applications are highlighted using this technology. Current applications include identifying species, identifying bodily fluids, and determining smoking status. We also review progress in potential applications for the future, including research on distinguishing monozygotic twins, detecting alcohol and drug abuse, and other phenotypic characteristics such as diet and body mass index. Overall, the versatility of the pyrosequencing technologies renders it a useful tool in forensic genomics. (Publisher abstract provided)
Downloads
Similar Publications
- Identification of Cadaveric Liver Tissues Using Thanatotranscriptome Biomarkers
- Experimental and Numerical Investigation of Gypsum Calcination under Fire Exposure
- Raman Spectroscopy and Chemometrics for Forensic Bloodstain Analysis: Species Differentiation, Donor Age Estimation, and Dating of Bloodstains