NCJ Number
213739
Journal
Journal of Forensic Sciences Volume: 51 Issue: 2 Dated: March 2006 Pages: 274-281
Date Published
March 2006
Length
8 pages
Annotation
This study produced a proposal for the allele nomenclature of six polymorphic short tandem repeat (STR) loci (PEZ3, PEZ6, PEZ8, PEZ10, FHC2161, and FHC2328) for canine genotyping.
Abstract
The standardization presented in this study, in addition to recently published results, make available 21 canine-specific STR markers. Further steps in determining a standard set of loci sufficient for canine individualization in forensics and the development of allelic ladders for each marker must be taken into consideration. The use of a standard DNA sample generally available as a control could be useful. In order to cover commonly and rarely occurring alleles, a selection of homozygous and heterozygous animals were analyzed and subjected to sequence studies. The alleles consisted of simple tri-nucleotide and tetra-nucleotide repeat patterns as well as compound and complex repeat patterns. Several alleles that revealed the same fragment size but different repeat structures were found. The allele designation described was adapted to the number of repeats, including all variable regions within the amplified fragment. In a second step, the most commonly occurring alleles were added to an allelic ladder for each marker, which permitted a reliable typing of all alleles that differed in size. A total of 142 unrelated dogs from surrounding municipal animal shelters, private households, and police dogs were analyzed. The data were added to a population database that provides allele frequencies for each marker. 1 table, 6 figures, and 34 references