NCJ Number
233923
Journal
Forensic Science International: Genetics Volume: 5 Issue: 1 Dated: January 2011 Pages: 57-63
Date Published
January 2011
Length
7 pages
Annotation
The authors of this paper tested the Powerplex S5 in comparison to the Powerplex ES in forensic casework for problematic samples with degraded DNA or PCR inhibitors.
Abstract
The new Powerplex S5 Mini STR-System from Promega with the four provided STR loci D18S51, D8S1179, TH01 and FGA as well as the Amelogenin marker (PCR products ranging from 80 to 220 bp not considering the longer FGA fragments) is designed as a screening tool especially in difficult casework samples. To test its suitability the authors amplified highly degraded DNA from casework samples, which had shown no or only poor results in analyses with the Powerplex ES kit, as well as artificially degraded DNA or DNA samples containing PCR inhibitors. Despite a tendency for allelic drop-ins in the amplification of highly degraded DNA the Powerplex S5 kit was a reliable tool for the analysis of casework samples with degraded DNA which gave better results than the Powerplex ES kit in 64 percent of analyzed swabs. Furthermore, it was especially suitable for the investigation of formalin fixed tissue, tissue samples showing advanced putrefaction or telogen hair samples. However, there was no strict relation between positive Powerplex S5 results and amplification success with the Powerplex ES kit. (Published Abstract) 2 tables, 5 figures, and 15 references