NucleoSpin DNA Clean-Up XS Kit for the Concentration and Purification of Genomic DNA Extracts: An Alternatives to Microdialysis Filtration

• NCJ Number: 234618
• Journal: Forensic Science International: Genetics Volume: 5 Issue: 3 Dated: June 2011 Pages: 226-230
• Author(s): William R. Hudlow; Robert Krieger; Markus Meusel; Joshua C. Sehhat; Mark D. Timken; Martin R. Buoncristiani
• Date Published: June 2011
• Length: 5 pages
• Publication Format: Article
• Publication Type: Research (Applied/Empirical)
• Language: English
• Country: United States of America

Annotation

This article reports on an optimized method for post-extraction DNA concentration and clean-up.

Abstract

This article discusses an optimized method to concentrate and clean up fluid extracts from the organic extraction of DNA from biological samples as an alternative to standard microdialysis filtration. The new method uses NucleoSpin XS silica columns to concentrate and clean the extracts. The method can be used both with standard organic extraction and dithiothreitol (DTT)-based differential extraction methods without making any modifications to the methods prior to concentration and cleanup. This article reports on the tests of the silica-binding method which uses a silica-membrane with a reduced effective membrane diameter. The reduced diameter membrane allows for low elution volumes making it attractive for use in low yield samples that are often encountered in forensic samples. The tests found that the silica-binding method could be used effectively as an alternative to the standard microdialysis filtration methods. Figures, table, and references