New Triplex STR System Without Irregular Alleles by Silver Staining and its Potential Application to Forensic Analysis

• NCJ Number: 188951
• Journal: Journal of Forensic Sciences Volume: 46 Issue: 3 Dated: May 2001 Pages: 448-452
• Author(s): Takashi Yoshimoto B.S.; Toshimichi Yamamoto Ph.D.; Rieko Uchihi Ph.D.; Keiji Tamaki M.D.; Xiu-Lin Huang M.D.; Masaki Mizutani M.D.; Miwa Tanaka B.D.S; John A. L. Armour B.M.; Yoshinao Katsumata M.D.
• Date Published: May 2001
• Length: 5 pages
• Publication Format: Article
• Publication Type: Report (Technical)
• Language: English
• Country: United States of America

Annotation

This study describes a new triplex short tandem repeat system for analyzing DNA.

Abstract

In order to increase the discriminating power of DNA analysis in forensic science, the study designed a new triplex short tandem repeat (STR) using three novel STR loci previously reported: D14S299 (wglc5), D15S233 (wgld1), and 9q2h2. The new system, designated a CDH triplex system, showed a high discriminating power, especially in Caucasians. The system is composed of three STR loci showing only regular tetranucleotide repeat alleles. The study enlarged the databases mainly of Japanese using this system, and compared them with databases of Caucasian and Chinese. Since all three loci have only regular tetranucleotide repeat alleles, each with high discriminating power, and have non-overlapping allele size ranges, the system can accurately type their alleles by electrophoresis on a denaturing polyacrylamide gel followed by silver staining in which the initial cost performance is lower than the fluorescent format. The study concludes that the CDH triplex system appears to be useful as a supplementary tool in forensic practice. Figure, tables, references