New Optimized DNA Extraction Protocol for Fingerprint Deposited on a Special Self-Adhesive Security Seal and Other Latent Samples Used for Human Identification

• NCJ Number: 236197
• Journal: Journal of Forensic Sciences Volume: 56 Issue: 5 Dated: September 2011 Pages: 1235-1240
• Author(s): Julieta Kopka, Ph.D.; Monita Leder, Ph.D.; Stella M. Jaureguiberry, M.Sc.; Gottfried Brem, M.D., D.V.M.; Gabriel O. Boselli, Ph.D.
• Date Published: September 2011
• Length: 6 pages
• Sponsoring Agency: BioSystems S.A.; Nexttec GmbH
• Publication Format: Article
• Publication Type: Test/Measurement; Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

The aim of this study was to evaluate a new kit, Fingerprint DNA Finder (FDF Kit).

Abstract

Obtaining complete short tandem repeat (STR) profiles from fingerprints containing minimal amounts of DNA, using standard extraction techniques, can be difficult. The aim of this study was to evaluate a new kit, Fingerprint DNA Finder (FDF Kit), recently launched for the extraction of DNA and STR profiling from fingerprints placed on a special device known as Self-Adhesive Security Seal Sticker and other latent fingerprints on forensic evidentiary material like metallic guns. The DNA extraction system is based on a reversal of the silica principle, and all the potential inhibiting substances are retained on the surface of a special adsorbent, while nucleic acids are not bound and remain in solution dramatically improving DNA recovery. DNA yield was quite variable among the samples tested, rendering in most of the cases (greater than 90 percent) complete STR profiles, free of PCR inhibitors, and devoid of artifacts. Even samples with DNA amount below100 pg could be successfully analyzed. (Published Abstract)