Modified Midi- and Mini-Multiplex PCR Systems for Mitochondrial DNA Control Region Sequence Analysis in Degraded Samples

• NCJ Number: 246108
• Journal: Journal of Forensic Sciences Volume: 58 Issue: 3 Dated: May 2013 Pages: 738-743
• Author(s): Na Young Kim, M.S.; Hwan Young Lee, Ph.D.; Sun Joo Park, Ph.D.; Woo Ick Yang, Ph.D.; Kyoung-Jin Shin, Ph.D.
• Date Published: May 2013
• Length: 6 pages
• Publication Format: Article
• Publication Type: Report (Technical); Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

Two multiplex polymerase chain reaction (PCR) systems (Midiplex and Miniplex) were developed for the amplification of the mitochondrial DNA (mtDNA) control region.

Abstract

Two multiplex polymerase chain reaction (PCR) systems (Midiplex and Miniplex) were developed for the amplification of the mitochondrial DNA (mtDNA) control region, and the efficiencies of the multiplexes for amplifying degraded DNA were validated using old skeletal remains. The Midiplex system consisted of two multiplex PCRs to amplify six overlapping amplicons ranging in length from 227 to 267 bp. The Miniplex system consisted of 3 multiplex PCRs to amplify 10 overlapping short amplicons ranging in length from 142 to 185 bp. Most mtDNA control region sequences of several 60-year-old and 400-500-year-old skeletal remains were successfully obtained using both PCR systems and consistent with those previously obtained by monoplex amplification. The multiplex system consisting of smaller amplicons is effective for mtDNA sequence analyses of ancient and forensic degraded samples, saving- time, cost, and the amount of DNA sample consumed during analysis. Abstract published by arrangement with John Wiley & Sons.