NCJ Number
213749
Journal
Journal of Forensic Sciences Volume: 51 Issue: 2 Dated: March 2006 Pages: 357-360
Date Published
March 2006
Length
4 pages
Annotation
This paper describes a new, rapid, robust, and inexpensive assay that simultaneously genotypes the four Duffy-based single-nucleotide polymorphisms (SNPs)--nt--33, 125, 265, and 298--using a minisequencing multiplex reaction; also reported are the sensitivity of Duffy and ABO minisequencing assays and their performance on biological stains in paternity testing cases and data from an Italian population sample (Bologna).
Abstract
In the Italian population sample (n=60), the study found 4 alleles and 7 genotypes for the Duffy and 6 alleles and 16 genotypes for the ABO systems. The lower limit for reproducible results was 200 pg DNA, with a range of up to 10 ng and an optimum at 1 ng. All of the 16 paternity tests were consistent with parentage, and 2 out of 4 inconsistencies with parentage cases were excluded by 1 or more SNPs. Although Duffy and ABO SNP typing produced less information than most current forensic tests, their robustness, limited population distribution of FY*Fy type and the sensitivity of the minisequencing technology indicate that these markers can be useful in selected forensic applications. In addition to the 60 unrelated volunteers in Bologna, 7 Masai from Kenya tested as controls. Duffy serotyping of samples was performed by hemagglutination methods with commercially available anti-Fya and anti-Fyb. Cotton cloth soaked in 0.5 ml saliva or semen and aged bloodstains that ranged from 4 to 13 years old with known phenotype were extracted by the QIAamp DNA micro-kit. ABO and Duffy assays were applied in 20 paternity cases. 4 tables, 2 figures, and 17 references