Improved Analysis of DNA Short Tandem Repeats with Time-of-Flight Mass Spectrometry Final Report

• NCJ Number: 179419
• Author(s): John M. Butler; Christopher H. Becker
• Date Published: 1999
• Length: 164 pages
• Grant Number(s): 97-LB-VX-0003
• Sponsoring Agency: National Institute of Justice (NIJ)
• Sale Source: National Institute of Justice/NCJRS; NCJRS Photocopy Services
• Publication Format: Document
• Publication Type: Report (Technical)
• Language: English
• Country: United States of America

Annotation

This report describes new technology for rapid and accurate analysis of DNA short tandem repeat (STR) markers using time-of-flight mass spectrometry.

Abstract

The report includes: (1) Executive Summary (descriptions of Gene Trace Systems and Mass Spectrometry, Short Tandem Repeats and Single Nucleotide Polymorphisms and Papers Published and Presentations Made); (2) Project Description and Grants; (3) Scope and Methodology (Assay Development and Primer Testing, Sample Cleanup and Mass Spectrometry, Sample Genotyping, Comparison Tests With ABI 310); (4) Results and Discussion for STR Analysis by Mass Spectrometry (Marker Selection and Feasibility Studies, Multiplex STR Work, Comparison Tests Between ABI 310 and Mass Spec Results, PCR Issues, Analytical Capabilities of Mass Spec); (5) Results and Discussion for Multiplex SNPs (Mitochondrial DNA Work, Y Chromosome Work); and (6) Conclusions and Implications of Findings. Notes, references, figures, tables