Genetic Profiles and Sex Identification of Found-Dead Wolves Determined by the use of an 11-Loci PCR Multiplex

• NCJ Number: 229901
• Journal: Forensic Science International: Genetics Volume: 4 Issue: 2 Dated: February 2010 Pages: 68-72
• Author(s): Barbara van Asch; Cintia Alves; Liliana Santos; Raquel Penheiro; Filipe Pereira; Leonor Gusmao; Antonio Amorim
• Date Published: February 2010
• Length: 5 pages
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: Ireland

Annotation

This article discusses genotyping between wolves and dogs.

Abstract

A previously described canine-specific 9-STR multiplex, now including two markers for sex determination, was tested for the genotyping of 23 wolves from Northern and Central Portugal. The samples were collected at necropsies and presented varying states of preservation. Complete profiles were obtained in 74 percent of the samples, partial profiles in 22 percent, and one completely null profile. This survey revealed 15 alleles not previously described in dogs, distributed among 6 STR loci. It is shown that this genotyping system, previously tested in domestic dogs, can be reliably used for obtaining complete genetic profiles in wolves with a matching probability of 2.45 x 10 (to the - 9 power) and compatible sex identification, even in suboptimal samples. Moreover, a population structure analysis using the observed genotypes revealed that this multiplexed 11-loci panel may potentially be used for discriminating between wolves and dogs. Tables, figures, and references (Published Abstract)