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Further Study to Investigate the Effect of Fingerprint Enhancement Techniques on the DNA Analysis of Bloodstains

NCJ Number
177856
Journal
Journal of Forensic Identification Volume: 49 Issue: 4 Dated: July/August 1999 Pages: 357-376
Author(s)
Claude Roux; Karen Gill; Julie Sutton; Chris Lennard
Date Published
1999
Length
20 pages
Annotation
This study investigated the effect of common and well- established fingerprint-enhancement techniques on the subsequent DNA analysis of items potentially bearing both fingerprints and biological evidence.
Abstract
Bloodstains of varying ages were prepared on different surfaces, and various fingerprint-enhancement techniques were applied to the samples. DNA typing was performed by using PCR amplification (D1S80 and CTT system). The results indicate that magnetic powder, multimetal deposition (MMD), and ultraviolet (UV) radiation are not recommended for use in a sequence of analyses that involves DNA typing. Strong white light, white and aluminum fingerprint powders, physical developer (PD) after 1,8- diaza-9-fluorenone (DFO), PD after ninhydrin with cadmium (Cd) salt treatment, and cyanoacrylate with gentian violet or Ardrox stains may be used successfully in a sequence of analyses that involves DNA typing. Ninhydrin with secondary metal salt treatment, DFO, amido black, diaminobenzidine (DAB), black powder, Stickyside Powder, cyanoacrylate with rhodamine stain, and luminol may be used before DNA analysis, but care must be taken to ensure that sufficient DNA is extracted and analyzed. 2 tables, 5 figures, and 11 references