Forensic Typing of Autosomal SNPs with a 29 SNP-Multiplex--Results of a Collaborative EDNAP Exercise

• NCJ Number: 223106
• Journal: Forensic Science International: Genetics Volume: 2 Issue: 3 Dated: June 2008 Pages: 176-183
• Author(s): J. J. Sanchez; C. Borsting; K. Balogh; B. Berger; M. Bogus; J. M. Butler; A. Carracedo; D. Syndercombe Court; L. A. Dixon; B. Filipovic; M. Fondevila; P. Gill; C. D. Harrison; C. Hohoff; R. Huel; B. Ludes; W. Parson; T. J. Parsons; E. Petkovski; C. Phillips; H. Schmitter; P. M. Schneider; P. M. Vallone; N. Morling
• Date Published: June 2008
• Length: 8 pages
• Contract Number(s): G6RD-CT-2002-00844
• Sponsoring Agency: European Cmssn; Ellen and Aage Andersen Foundation
• Publication Format: Article
• Publication Type: Report (Technical)
• Language: English
• Country: Ireland

Annotation

This paper reports on the results of an interlaboratory exercise on typing of autosomal single nucleotide polymorphisms (SNPs) for forensic genetic investigations in crime cases.

Abstract

The results of the collaborative exercise were surprisingly good and showed that SNP typing with single base extension (SBE), capillary electrophoresis, and multicolor detection methods could be developed for forensic genetics. The total SNP locus dropout rate was 2.8 percent, and more than 50 percent of the dropouts were observed with the poor-quality sample. The overall rate of discrepant SNP allele assignments was 2.0 percent. Two laboratories reported 60 percent of all the discrepancies. Two laboratories reported all 29 SNP alleles in all 10 positive samples correctly. The European DNA Profiling Group (EDNAP), a working group under the International Society for Forensic Genetics (ISFG) organized the exercise. A total of 11 European and 1 U.S. forensic genetic laboratories tested a subset of a 52 SNP-multiplex PCR. The 52 SNPs are detected in two separate SBE multiplex reactions with 29 and 23 SNPs, respectively, using SNaPshot kit, capillary electrophoresis, and multicolor fluorescence detection. For practical reasons, only the 29 SBE multiplex reaction was conducted by the participating laboratories. A total of 11 bloodstains on FTA cards, including a sample of poor quality and a negative control, were sent to the laboratories, together with the essential reagents for the initial multiplex PCR and the multiplex SBE reaction. 3 tables, 2 figures, and 13 references