NCJ Number
222070
Journal
Forensic Science International: Genetics Volume: 2 Issue: 2 Dated: March 2008 Pages: 104-107
Date Published
March 2008
Length
4 pages
Annotation
This study compared and evaluated two different ancient DNA extraction methods: magnetic separation and silica binding.
Abstract
The efficiency rate of these two DNA extraction methods seems to be identical. The silica binding method has the benefit of relative simplicity, but the magnetic separation technique also has advantages. It is possible to reuse the extract several times for different loci, and it is possible to concentrate all extracted DNA from one locus into one PCR. When working with ancient DNA, the main problem stems to some degree from the low amount of starting molecules, and the presence of PCR inhibitors, but also includes endogenous DNA damage and fragmentation. This study presents results from an evaluation of two ancient DNA extraction methods. The method relying on silica binding columns is commonly used on museum material of moderate age, as well as DNA from prehistoric tissue. The other method is based on a purification concept that has yet not been widely exploited in ancient DNA extractions, purifying ancient DNA with magnetic separation. Even if the two methods seem to be equal in efficiency, all the characteristics of the fishing method have yet to be fully studied. Tables, references