NCJ Number
182156
Journal
Journal of Forensic Sciences Volume: 45 Issue: 2 Dated: March 2000 Pages: 354-380
Date Published
March 2000
Length
27 pages
Annotation
This study determined the effect of seven blood enhancement reagents on the subsequent Profiler Plus fluorescent short tandem repeat (STR) DNA analysis of fresh or aged bloody fingerprints deposited on various porous and nonporous surfaces.
Abstract
Amido Black, Crowle's Double Stain, 1,8-diazafluoren-9-one (DFO), Hungarian Red, leucomalachite green, luminol, and ninhydrin were tested on linoleum, glass, metal, wood, clothing, and paper. Preliminary experiments were designed to determine the optimal blood dilutions to use to ensure a DNA typing result following chemical enhancement. A 1:200 blood dilution deposited on linoleum and enhanced with Crowle's Double Stain generated enough DNA for one to two rounds of Profiler Plus PCR amplification. Both luminol and Amido Black produced excellent results on both porous and nonporous surfaces, but Crowle's Double Stain failed to produce any results on porous substrates. Hungarian Red, DFO, Leucomalachite green, and ninhydrin showed lower sensitivities. Study findings show that significant evidence can be obtained from fresh or aged bloody fingerprints applied to a variety of absorbent and nonabsorbent surfaces that are exposed to different enhancement chemicals for short or long periods of time. It also reaffirms that PCR STR DNA typing procedures are robust and provide excellent results when used in concert with fluorescence-based detection assays after fingerprint identification has occurred. 12 tables, 14 figures, and 67 references