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Evaluation of a novel material Diomics X-Swab for collection of DNA

NCJ Number
255494
Journal
Forensic Science Genetics International Volume: 12 Dated: 2014 Pages: 192-201
Author(s)
P. L. Marshall; M. Stoljarova; B L. Larue; J. L. King; B. Budowle
Date Published
2014
Length
10 pages
Annotation

In the current study, the ability to recover DNA from X-Swab material and success in STR typing were compared with the Copan 4N6FLOQSwab™ (Brescia, Italy), a device which uses a proprietary flocked-swab technology to maximize DNA collection and elution efficiency.

Abstract

Success of DNA typing is related to the amount of target material recovered from an evidentiary item. Generally, the more DNA that is recovered, the better the chance is of obtaining a typing result that will be robust and reliable. One method of collecting stain materials is by swabbing. Recovery of DNA from some commercially available swabs is not an efficient process. The X-Swab™ (Diomics Corporation, La Jolla, CA) is a unique bio-specimen collection material with highly absorptive properties and can be dissolved during certain extraction conditions; therefore, more DNA may be collected from a substrate and be released from the swab matrix compared to other swabs. In the current study, both types of swabs were impregnated with known amounts of DNA and body fluids and allowed to air dry. In addition, blood was placed onto glass slides, allowed to dry, and collected using both types of swabs. DNA recovery was assessed by DNA quantitation and by STR typing. Results suggested that X-Swab material yielded greater DNA recovery, particularly of low quantity samples (defined as diluted neat samples), compared with the 4N6FLOQSwab. Results also indicated that X-Swab material itself improves the yield of PCR products. (publisher abstract modified)